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After treatment, tissues were maintained in 2% FBS DMEM until the 10th days, the medium was changed every 3 days
This mutation leads to the absence of functional leptin, causing profound obesity, hyperphagia, and metabolic dysregulation beginning early in development (21)
When combined with appropriate lifestyle changes, it supports both significant weight loss and improved cardiometabolic health
Statements Author contributions SM: Conceptualization, Writing original draft
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