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Crystals of ShHTL1 were grown by mixing 0.45 L of purified ShHTL1 protein solution (15.0 mg mL 1 ) with 0.45 L of reservoir solution containing 100 mM Tris (pH 8.5), 150 mM MgCl 2 , and 23% (w/v) PEG6000 and allowing the drop to equilibrate at 5 C
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This is what pharmacologists call an orphan receptor a receptor with no identified endogenous ligand
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