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Zhou, S., Han, L
To verify the importance of pocket size for ligand recognition of ShHTLs, a double mutation T190F/C194F and a triple mutation L124F/T190F/C194F of ShHTL7 were generated to introduce large side-chain amino acids to reduce the pocket size by partially mimicking ShHTL3
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The levels of Fe 2+ in each of tissue sample were calculated based on this standard curve
L-arginine is considered a semi-essential amino acid
