Uniform gain and exposure settings in the Nikon NIS Imaging Software with identical objective lens magnification of 40 with a numerical aperture of 0.75 were applied to all sections to ensure consistency in image intensities and magnification
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The geolocations of the posts are focused on cities, although this could simply reflect the larger human and dog populations in these areas
Subsequently, they received intravenous injections of PBS, Ru(II)-OH, NP2, or NP4 (at a dosage of 2 mg/kg Ru) on days 0, 3, 6, and 9, respectively