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The lysate was subsequently centrifuged (12000 g, 4 C, 5 min), denatured, analysed by SDSPAGE, and transferred to PVDF membranes (IPVH15150, Millipore)

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If itching intensifies after switching sites properly, your provider can adjust injection technique or explore whether your immune genes (testable via PlexusDx Precision Peptide Genetic Test) predispose you to stronger local reactions