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After centrifugation at 4,000 g for 10 min, the supernatants were used for assays, and absorbance at 520 nm was measured using a microplate reader

In clinical trials, discontinuation rates due to gastrointestinal adverse effects ranged from 3-7%, indicating that whilst most patients can tolerate these symptoms, they can be sufficiently troublesome to necessitate treatment cessation in some cases

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This targeted approach offers potential advantages over traditional testosterone replacement therapy by addressing root causes of age-related hormonal decline rather than simply supplementing exogenous hormones

These symptoms were also most frequently reported as most bothersome and important to improve.All participants reported 1 or more symptoms included in the FCS-SIS within the 7-day recall period, understood the items, and stated that at least one improvement would be meaningful